Clinical application of serum vitamin B12 assay.
نویسنده
چکیده
منابع مشابه
Reduction of Serum Vitamin B12 Levels among the Female Patients with Breast Cancer (Case Study: Sulaimania city- Iraq)
Background and objectives: Breast carcinoma is one of the most common malignant diseases among women worldwide. In Iraq there are noticeable elevation in incidence rates and prevalence of advanced stages of breast cancer. Cobalamin (vitamin B12) is essential micronutrient involved in one carbon metabolism and DNA methylation, which affects cancer. All of these may be change in breast cancer. Th...
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We describe a vitamin B12 radioassay in which oyster toadfish (Opsanus tau) serum is used as the binding protein. The serum is quite stable and has a high capacity and high binding affinity (K greater than 10(12) liter/mol) for vitamin B12. The binding is not significantly affected by temperature, the presence of denatured proteins, or the amount of vitamin B12 present. The radioassay is reprod...
متن کاملComparison of two methods for radioassay of vitamin B12 in serum.
A reference radioassay for vitamin B12 in serum, in which human serum is used as binder and DEAEcellulose is used to separate free and bound B12, was compared (194 sera) with a packaged kit (“Phadebas B12 Test”), in which intrinsic factor is used as binder and poiysaccharide for separation. The correlation coefficient was 0.949. With the kit, added B12 was identifiable, proportional dilution ch...
متن کاملVitamin B12 in human blood and serum. I. Comparison of microbiologic assays using normal subjects.
HE MICROORGANISMS used for Vitamin B12 (cyanocobalamin) assay are LaCtobaciUus lewhmanniz (1), Euglena gracilis (2), Ochromonas maihamensis (3-5), and a mutant strain of Escherichia COli (E. coli 113-3) (6). Improvements in protozoan assays for B12 show such assays to be more sensitive and specific than bacterial assays (2, 7, 8). Ochromonas maihamensis is highly specific for true forms of B12 ...
متن کاملExtraction of serum vitamin B12 for radio-isotopic and Lactobacillus leichmannii assay.
The protein precipitates discarded during the extraction process of the Lactobacillus leichmannii vitamin B12 assay have been shown to contain significant amounts of vitamin B12. This loss of vitamin B12 provide a satisfactory explanation for many of the descrepancies between the serum vitamin B12 values obtained by the L. leichmannii method and the radio-isotopic method of Raven et al (1969). ...
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 34 4 شماره
صفحات -
تاریخ انتشار 1981